There was a lot of high-quality golf played throughout the day, and two aces were recorded along the way. Jack Yule managed a hole-in-one on the 4th hole at Gog Magog, while John Ambridge managed the same feat at Moor Park, where he was club captain in 2023.
Robert Harrhy was the class of the field at Frilford Heath, posting the lowest score of the day with a six-under 66 that saw him finish three clear of his nearest competitors.
In total almost 2,000 players entered Regional Qualifying, with former Premier League footballers Jimmy Bullard and Peter Odemwingie among those who were unable to progress on this occasion.
Bullard shot a three-over-par 74 at Rochester & Cobham Park, while Odemwingie posted an 82 at Enville.
Final Qualifying will take place on Tuesday 1 July at Burnham & Berrow, Dundonald Links, Royal Cinque Ports and West Lancashire.
The 153rd Open will take place at Royal Portrush from 13 to 20 July.
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Players to qualify at Regional Qualifying
Bearwood Lakes: John Gough (-3), Thomas Plumb (-3), Stephen Ferreira (-2), Ian Fritz (-1), Curtis Knipes (-1), Reggie Fear (Even), Joey Lussier (Even), Charlie Townsend (+1), Kris Kim (+1).
Caldy: Peter Finch (-1), Cameron Walker (-1), Tim Ford (Even), Jon Beesley (Even), Max Ranson (Even), George Holland (+1), Caolan Burford (+1), Sam Johnston (+2), Thomas Matthews (+2).
Craigielaw: Jack McDonald (Even), Matthew Wilson (Even), Jordan Sundborg (+2), Euan MacDonald (+2), Oliver Mukherjee (+2), Archie Finnie (+2), Ross Munro (+2), Joe Bryce (+2).
Enville: Jacob McGoldrick (-5), OJ Farrell (-4), Ryan Brooks (-4), Dani Hoof (-4), James Claridge (-3), James llsley (-3), Luke Blocksidge (-3), Jack South (-2), Joel Tarry (-1).
Ferndown: Jake Burnage (-4), Robert Dinwiddie (-3), Joe Retford (-2), Tom Sloman (-2), Finbar Kane (-2), Andrew Richardson (-2), Jake Bolton (-1), Jack Singh Brar (-1), Craig Sutherland (-1).
Frilford Heath: Robert Harrhy (-6), Will Tate (-3), Matthew Hibbs (-3), Harry Richardson (-3), Jamie Dick (-2), Ollie Brimfield (-2), Max Nicholls (-2), William Robbins (-2), Alex Ching (-1).
Fulford: Huey Cunningham (-3), Habib Khan (-3), Michael Hay (-2), Ashton Turner (-1), Charlie Box (-1), Joe Knox (-1), Ollie White (-1), Ashley Mansell (Even), Jack Peters (Even).
Gog Magog: Ed Featherstone (-3), Timmy Moncur (-3), Jack Hawksby (-3), Giles Evans (-1), Max Weaver (-1), Max Hopkins (-1), James Jankowski (-1), Callum Hilton (-1), Sam Byford (Even).
Hesketh: Jamie van Wyk (-2), Nick Marsh (+1), Tom Hughes (+1), Luke Harries (+2), Joe Emery (+2), Greg Holmes (+2), Lewis Pownell (+3), Rhys Nevin (+3), Matt Lever (+4).
Kilmarnock (Barassie): Liam Johnston (-3), Michael Stewart (-2), Scott Henry (-2), James Donaldson (-1), Marc Warren (-1), Adam Chapman (Even), Mitchell Cowie (Even).
Lindrick: Frazer Jones (-3), Bailey Gill (-2), Oliver Sullivan (-1), Julian Wood (-1), Sam Potter (Even), Josh Crumplin (Even), Rob Harris (Even).
Moor Park: Adam Batty (-3), Habebul Islam (-3), Michael Vonbank (-3), Ville Virkkala (-3), Matt Gauntlett (-2), Arran Kanth (-2), Niklas Adank (-2), Nick Ward (-2), Owen Benson (-1).
North Hants: Freddie Turnell (-4), Robert Wheeler (-2), Lawrence Courtney (-1), Harry Ellis (-1), Harry Bowness (Even), Greg Hurley (Even), Josh McCartain (Even), Joshua Millington (Even), Charlie Preston (+1).
Rochester & Cobham Park: Max Jordan (-3), Jonathan Exon-Taylor (-2), Oliver Lewis-Perkins (-2), Finn Lord (-2), Ant Tarchetti (-2), Jack Lee (-1), Carl Burrows (-1), Raul Gomez (-1), Sam Smitherman (-1).
The Island: Paul McBride (-3), Keith Shannon (-2), Rowan Lester (-1), Finlay Eager (-1), Ronan Mullarney (-1), Brendan McCarroll (-1), Alex Hietala (+1), Ruaidhri McGee (+1), Niall Kearney (+2), Alex Maguire (+2).